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Bulk anti-CD3e In Vivo Antibody – Low Endotoxin (500A2)
Product Benefits:
ichorbio’s anti-CD3e In Vivo Antibody – Low Endotoxin (500A2) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio offers Amazon vouchers or donations to the NC3Rs for reviews of this product: click here for more information. ichorbio: the best antibodies for in vivo research.
Target:
CD3e
Clone:
500A2
Isotype:
Syrian Hamster IgG2a
Other Names:
T3/Leu-4 epsilon chain, CD3 epsilon chain, CD3e
Host:
Syrian Hamster
Species Reactivity:
Mouse
Specificity:
Anti-CD3e In Vivo Antibody – Low Endotoxin (500A2) recognizes an epitope on Mouse CD3ε
Purification Method:
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution:
The CD3 complex is present on thymocytes and mature T-lymphocytes of all mouse strains tested.
Background:
CD3 subunits gamma, delta and epsilon are required for proper assembly, trafficking and surface expression of the TCR complex.
Immunogen:
Mouse T-cell receptor
Concentration:
1.0 mg/ml
Formulation:
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.
Purity:
>95% by SDS-PAGE and HPLC
>98% by SDS-PAGE and HPLC
Endotoxin:
<1.0 EU/mg as determined by the LAL method
≤ 0.75 EU/mg as determined by the LAL method
Aggregation:
Aggregation level ≤ 5%
Aggregation level ≤ 1%
IMPACT Pathogen Test:
We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our hamster antibodies are tested for:Mycoplasma sppMycoplasma pulmonisPneumonia virus of miceKilham’s rat virusToolan’s H1 virusHamster parvovirusLymphocytic choriomeningitisMinute virus of miceTheiler’s murine encephalomyelitis virusSendai virusReovirus 3Hantaan virus
Storage:
anti-CD3e In Vivo Antibody – Low Endotoxin (500A2) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Applications:
Functional Assays, Flow Cytometry, IHC (Frozen)
Application Notes:
Functional Assay: Binding of Hamster Anti-Mouse CD3ε (Clone 500 A2) to CD3 initiates the intracellular biochemical pathway resulting in cellular activation and proliferation.Each investigator should determine their own optimal working dilution for specific applications.
Use:
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Related Antibody:
Antibodies against the same target:
Anti-CD3e In Vivo Antibody – Ultra Low Endotoxin [145-2C11] (ICH1041UL), Anti-CD3e In Vivo Antibody – Low Endotoxin [145-2C11] (ICH1041)
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